Services

Quick Links for Getting Started

Consider your optimal cell density for your cell type:

• The Agilent Life Science Publication Database can assist you in locating publications which use your cell type to help find optimal cell density.
• We recommend testing at least 3 concentrations: suspected optimal (X), 0.5X, and 2X.

Plan your experiment and set up your plate layout

• If you’ve never done Seahorse on your cells before, your first experiment should be to optimize the cell number.
• You may also want to plan an experiment to optimize the FCCP concentration for your cells if you are running a Mito Stress Test or the Cell Energy Phenotype Test.
• We recommend you test at least 3-6 replicates of each sample.

Example Plate Map for Cell Number Optimization (0.5X, 1.0X, 2X, 4X)

Review your plan with the Core and set a date to run

• Please contact us (Qing Shi and Erika Rezeli) to set up a time to discuss your experimental plan and reserve your time slot. The AMP Core operates Seahorse services Tuesday through Friday, with two run options per day: 9am-1pm or 1pm-5pm.

Request your service on iLab

Questions? Please see our Frequently Asked Questions page or contact us.

Agilent Contacts

• Seahorse Specialist Aurellia Whitmore: aurellia.whitmore@agilent.com
• Seahorse Applications Specialist Manish Bharadwaj: manish.bharadwaj@agilent.com
• Seahorse Technical Support Line: 800-227-9770, option 3, option 8