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Quick Links for Getting Started

Consider your optimal cell density for your cell type:

  • The Agilent Life Science Publication Database can assist you in locating publications which use your cell type to help find optimal cell density.
  • We recommend testing at least 3 concentrations: suspected optimal (X), 0.5X, and 2X.

Plan your experiment and set up your plate layout

  • If you’ve never done Seahorse on your cells before, your first experiment should be to optimize the cell number.
  • You may also want to plan an experiment to optimize the FCCP concentration for your cells if you are running a Mito Stress Test or the Cell Energy Phenotype Test.
  • We recommend you test at least 3-6 replicates of each sample.

Example Plate Map for Cell Number Optimization (0.5X, 1.0X, 2X, 4X)






Review your plan with the Core and set a date to run

  • Please contact us (Qing Shi and Erika Rezeli) to set up a time to discuss your experimental plan and reserve your time slot. The AMP Core operates Seahorse services Tuesday through Friday, with two run options per day: 9am-1pm or 1pm-5pm.

Request your service on iLab

Questions? Please see our Frequently Asked Questions page or contact us.

Agilent Contacts


Qing Shi

Research Specialist, Nutrition

Department of Nutrition
2215 Michael Hooker Research Center
Chapel Hill, NC 27599

(919) 966-7162

Research Specialist, Animal Metabolism Phenotyping Core

Erika Rezeli

Manager, Hursting Lab (Chapel Hill)

Michael Hooker Research Center
Chapel Hill, NC 27599

(919) 966-5590

Manager, Hursting Lab (Chapel Hill)