Services

Getting Started

Begin to plan your experiment 

Seahorse Metabolic Flux assays are highly dependent on having optimal cell density and cell health.  We recommend that your first experiment includes cell number optimization.

The Agilent Life Science Publication Database can assist you in locating publications which use your cell type to help find optimal cell density.

We recommend testing at least 3 cell concentrations: suspected optimal (X), 0.5X, and 2X.

Contact the Core to review your plan and book your run.

Please contact us (Qing Shi at qing_shi@med.unc.edu and Erika Rezeli at tuerika@email.unc.edu) to set up a time to discuss your experimental plan and reserve your booking.  The GEM: Metabolic Phenotyping Core operates Seahorse services Tuesday through Friday, with two run options per day: 9am start time or 1pm start time.

Request your service on iLab

Questions? Please see our Frequently Asked Questions page or contact us.

Example Plate Map for Cell Number Optimization (0.5X, 1.0X, 2X, 4X) and FCCP optimization (0-2µM)

Agilent Contacts

• Seahorse Specialist Aurellia Whitmore: aurellia.whitmore@agilent.com
• Seahorse Applications Specialist Manish Bharadwaj: manish.bharadwaj@agilent.com
• Seahorse Technical Support Line: 800-227-9770, option 3, option 8